【摘要】 目的制备针对藻毒素(MCLR)的多克隆抗体,并鉴定其特性。方法利用合成的免疫抗原MCKLH结合物,按常规方法免疫新西兰大白兔20周,经离心、硫酸铵沉淀法制得纯化的抗体,计算其亲和常数,用间接竞争法测定效价、抑制率IC50及特异性。结果纯化的MCLR抗血清效价大于25600,最低检出限为0.01ngml,IC在0.1～1ngml之间,与MCLW和MCLF的交叉反应率为0.1%～20%。更多还原

【Abstract】 Objective Production and characterization the polyantibodies against MCYST-LR.Methods microcystin-LR (MC-LR) was conjugated to KLH, then immune the rabbit by the routine method. Purify the antiserum by centrifugal and saturated sulfuric ammonium precipitated methods. Count the affinity constant and measure the titers, IC_ 50 Value and the sensitivity.Results The titers of the antiserum is over 25600. IC_ 50 value is about from 0.1ng/ml to 1ng/ml. The across-reactivity ratio to MC-LW and MC-LR is about from 0.1% to 20%.更多还原