【摘要】 探讨鼻黏膜给予少量IFN-γ对实验性自身免疫性重症肌无力(EAMG)鼻黏膜免疫耐受的逆转作用。在用自身抗原乙酰胆碱受体(AChR)和完全弗氏佐剂(CFA)免疫Lewis大鼠之前,通过鼻黏膜分别给予重组大鼠IFN-γ(5 000 U/只);AChR+IFN-γ或单独给予AChR,另外给予等量AChR的同时,腹膜注射IFN-γ(5 000 U/只)。评估不同组对EAMG发病的影响。可见,鼻黏膜单独给予AChR有效诱导EAMG免疫耐受,而同时给予AChR和IFN-γ与对照组(只给予PBS)相比扩大了T、B细胞对AChR的免疫应答,出现了与对照组相似的临床症状。相反,AChR+IFN-γi.p.不影响对EAMG的耐受,鼻黏膜单独给予IFN-γ对EAMG的临床症状没有影响。经不同途径给予IFN-γ对免疫耐受有不同的影响:鼻黏膜给予少量IFN-γ可以打破免疫耐受;而经腹腔给予IFN-γ不会影响免疫耐受。更多还原

【Abstract】 Reversal of nasal mucosa immune tolerance to experimental autoimmune myasthenia gravis(EAMG) with administration of minute amount of interferon-γ(IFN-γ) was investigated.Before Lewis rat was immunized with autoantigen acetylcholine receptor(AChR) and complete Freund’s adjuvant(CFA),recombinant rat IFN-5 000 U/rat);AChR+IFN-γ,or AChR alone,was given nasally respectively to Lewis rat to investigate whether nasally induced tolerance to EAMG could be influenced by nasal mucosa administration of cytokines.On other hand,one additional group of rats received intraperitoneally the same amount of AChR nasally in conjunction with IFN-γ(total 5 000 U/rat),thus to evaluate the effect of EAMG pathogenesis of each group.The results showed that AChR given alone nasally induced effective tolerance to EAMG whereas rats receiving AChR+IFN-γ by nasal route exhibited a similar disease pattern,and similarly escalated T and B cell responses to AChr as compared with control rates.In contrast,administration of IFN-γ i.p.together with AChR nasally did not affect the induction of tolerance to EAMG.IFN-γ given alone nasally did not affect clinical EAMG.Therefore,nasal tolerance can be modulated by nasal administration of minute amounts of IFN-γ.Nasal administration of certain cytokines with beneficial effects might broaden the effectiveness of applying nasal tolerance as a potential therapeutic means of preventing autoimmune disease.更多还原