【摘要】 对苏云金杆菌79007菌进行了生长条件的研究,获得了该菌最佳发酵培养基配方和最优发酵工艺。最佳培养基配方(g/100 mL):豆饼粉2.5,淀粉2.0,酵母粉2.0,玉米浆2.0,添加剂Ⅰ号0.1,葡萄糖1.0,KH2PO40.3,CaCO30.1,CaCl20.05,MgCl20.01,CuCl20.005,ZnSO40.002,CoCO30.005;15 m3发酵罐最优工艺:通气量1∶0.5~1.1,培养温度28~33℃,搅拌转速0~200 r/min。40 m3发酵罐连续4批试验表明,平均发酵效价6 053.5 IU/μL、平均晶体蛋白含量达0.5%,产品质量达到原粉优质品。更多还原

【Abstract】 Growing conditions of Bacillus thuringiensis "79007" were studied.The results showed that the optimized formula of medium（g/100 mL） and technology were as follow: soybean meal 2.5,cornstarch 2.0,yeast powder 2.0,corn syrup 2.0,additive I 0.1,glucose,1.0,KH₂PO₄ 0.3,CaCO₃ 0.05,MgCl₂ 0.01,CuCl₂ 0.005,ZnSO₄ 0.002,CoCO₃ 0.005,and the aeration in the 15 m³ fermentor tank 1∶0.5～1.1,at 28～33 ℃ with 0～200 rpm of mixing speed.Four batches of experiment in 40 m³ fermentor tank showed that the average valence was 6 035.5 IU/μL,and the average content of crystal protein was 0.5%,the product quality reached the top quality of original powder.更多还原