【摘要】 以农杆菌介导法建立的红曲霉T-DNA转化库为实验材料,采用抑菌圈法从5,000多个转化子中筛选出200株桔霉素突变子的候选菌株,用高效液相色谱法进一步筛选得到53株与出发菌株相比产桔霉素能力发生显著变化的突变子,其红曲中桔霉素含量介于0·04~154·57μg/g之间。进一步分析了突变子的红曲色价,发现突变子产桔霉素能力与产色素能力之间有一定的相关性。这些研究结果为进一步从分子水平上探讨红曲霉产桔霉素和色素等次生代谢产物之间的关系提供了材料和基础。更多还原

【Abstract】 200 citrinin mutants were screened with the inhibition zone method from the transformants library of Monascus ruber M-7 by Agrobacterium-mediate DNA transfer, which contains more than 5,000 transformants.Then 53 mutants, whose citrinin contents ranged from 0.04μg/g to 154.57μg/g in the red fermented rice (RFR), were achieved by high performance liquid chromatography (HPLC).Color values of RFR prepared by these mutants were also detected.The results showed that there was a positive correlation between the citrinin content and the color value among the mutants.These results provide materials and research bases for ferrther studying the relationship between the production of citrinin and pigment of Monascus ruber at molecular level.更多还原