【摘要】 采用PCR定点突变技术对睫状神经营养因子(CNTF)基因进行改造,获得了CNTF衍生物(CNTF-D)基因序列分析证明其核苷酸序列符合设计要求,并在大肠杆菌中获得高效表达,CNTF-D表达量超过40%,复性后,经Q-Sepharose HP纯化,其纯度超过95%,体内法测定发现能明显降低实验小鼠的体重。表明CNTF-D在体内具有良好的生物学活性,为下游开发奠定了基础。更多还原

【Abstract】 Based on the natural human CNTF gene,the mutated hCNTF gene was generated by site-specific mutagenesis.The gene was inserted into E.coli and confirmed by DNA sequencing.The high expression of CNTF-D has been achieved.The expression level of recombinant protein reached to more than 40%.The purity of CNTF-D was about 95% after denaturing and purifying by Q-Sepharose HP chromatography.The biological activity of CNTF-D was measured by mouse in vivo test.The results showed that this mutant protein could obviously reduce body weight of tested mouse.It laid a foundation in development of a new type of high efficient body weight reducing drug.更多还原