【摘要】 荧光标记的微管经激发光照射会引起微管的断裂,但其断裂过程及机理并不清楚.通过光镊研究了微管光敏断裂的动态过程.实验结果表明组成微管的原丝并不是同时断裂的,而是相继断裂最终导致微管的断裂.经过一系列生物学实验,确定了活性氧中的自由基是造成微管光敏断裂的主要因素.根据光镊研究的结果推测了自由基作用于微管的机理.更多还原

【Abstract】 It is reported that fluorescent microtubules may break under irradiation. However, the process and mechanism are not well understood. In this paper, we study the process of light-induced microtubule breaking by optical tweezers and find the breakup of protofilaments in microtubule is not simultaneous but proceeds step-by-step. After a series of biological experiments, we confirm that free radicals are the main reason for the photosensitive breaking of microtubule. According to the results of optical tweezers experiments, the mechanism of free radicals acting on microtubule is infered.更多还原