【摘要】 目的探讨二烯丙基二硫(DADS)对HL-60白血病细胞株的作用。方法采用MTT法观察DADS对体外培养的HL-60细胞的生长抑制;应用酶联免疫吸附剂测定(ELISA)法检测药物作用前后白血病HL-60细胞培养上清液中VEGF蛋白的含量。结果DADS对HL-60细胞具有明显的生长抑制效应,其对HL-60细胞的生长抑制率与药物浓度、时间有明显依赖关系,DADS(作用细胞72 h)浓度从0.625μg/ml增至20.0μg/ml,其对HL-60细胞的生长抑制率由16.7%增至71%,各实验组随时间增加抑制率明显增加(P<0.01);HL-60细胞中均有VEGF蛋白的分泌,与空白对照组相比DADS 3个浓度组(0.625,1.25,2.5μg/ml)作用HL-60细胞72 h均能下调VEGF蛋白的分泌(P<0.01)。结论DADS能明显抑制HL-60细胞的增殖;DADS可能通过抑制HL-60细胞VEGF的分泌发挥抗白血病的效应。更多还原

【Abstract】 Objective To investigate the effect of diallyl disulfide(DADS) on proliferation of HL-60 leukemic cells and to investigate the changes in secretion of vascular endothelial growth factor protein in HL-60 cells,thus exploring the mechanism of DADS against leukemia.Methods MTT was used to assay the growth of HL-60 cells treated with DADS.ELISA was used to study the secretion of VEGF protein in HL-60 cell lines treated with DADS.Results DADS significantly inhibited proliferation of HL-60 cells in a dose-and time-dependent manner(r>0.7,P<0.01).The inhibiting rate of HL-60 cells for 72 h increased from 16.7% to 71% with DADS increasing from(0.625) μg/ml to 20 μg/ml.VEGF was expressed in HL-60 cells.The secretion of VEGF protein could be downregulated by 0.625,1.25, and 2.5 μg/ml DADS-treated HL-60 cells for 72 hours.The differences between the DADStreated HL-60 cell groups and the control group were statistically significant (P<0.01);there were also statistically significant differences between the three DADS-treated HL-60 cells groups(P<0.05).Conclusion DADS effectively inhibits the proliferation of human leukemia cell line HL 60 cell;DADS exerts its anti-leukemia effects by reduction of the secretion of VEGF protein.更多还原