【摘要】 目的以无关个体为研究对象,研究中国藏族人群线粒体DNA D环区序列遗传多态性。方法应用PCR扩增产物直接测序法,对30名藏族无关个体线粒体DNA D-环区高变区Ⅰ(hypervariable regionⅠ,HVR-Ⅰ)进行测序分析。结果HVR-Ⅰ中404个核苷酸序列与Anderson相应序列比较共发现有51处突变,构成28种单倍型。藏族线粒体DNA HVR-Ⅰ基因差异度为0.9862,偶合概率为0.0466。结论线粒体DNA D环区序列多态性在法医学领域具有较高的应用价值。更多还原

【Abstract】 Objective To study the genetic polymorphisms of the mitochondrial DNA(mtDNA) D-loop region in Lhasa Tibetan population in China.Methods Sequence polymorphisms of 30 unrelated Tibetan mtDNA D-loop hypervariable regionsⅠwere determined by PCR and direct sequencing.Results Fifty-one polymorphic sites and 28 haplotypes were identified in the sequences of 404 nucleotides for HV-Ⅰ,compared with the reference sequence.The genetic diversity was calculated to be 0.9862,and the genetic identity was 0.0466.Conclusions Sequence polymorphism of mtDNA D-loop region is very useful in forensic practice as a marker for individual identification.更多还原