【摘要】 背景:强力霉素对结肠癌细胞的分化和抑制作用已有报道,但其对胃癌细胞的作用尚未见报道。目的:观察强力霉素对人胃癌细胞SGC-7901的生长抑制作用及其对基质金属蛋白酶(MMP)-2和基质金属蛋白酶组织抑制因子(TIMP)-2表达的影响,探索胃癌治疗的新方法。方法:采用不同浓度的强力霉素作用于胃癌细胞SGC-7901,以噻唑蓝(MTT)法测定其细胞毒作用;逆转录聚合酶链反应(RT-PCR)法半定量测定MMP-2和TIMP-2mRNA的表达;免疫组化法观察MMP-2蛋白的表达。结果:强力霉素可抑制胃癌细胞SGC-7901的生长,具有浓度和时间依赖性(P<0.01)。强力霉素可下调MMP-2mRNA和MMP-2蛋白的表达,上调TIMP-2mRNA的表达,具有浓度依赖性(P<0.05)。结论:强力霉素能抑制胃癌细胞SGC-7901的生长,其作用机制可能与下调MMP-2表达、上调TIMP-2表达有关。更多还原

【Abstract】 Background: Doxycycline could inhibit the proliferation of colonic cancer cells. However, its effect on gastric cancer cells has not been reported. Aims: To observe the inhibitory effect of doxycycline on the growth of human gastric cancer cells (SGC-7901) and the effect on expression of matrix metalloproteinase (MMP)-2 and tissue inhibitor of metalloproteinase (TIMP)-2 and to explore new therapeutic approach in the treatment of gastric cancer. Methods: SGC-7901 cells were treated with different concentrations of doxycycline and its growth inhibitory effect was detected by methyl thiazolyl tetrazolium (MTT) method. The mRNA expression of MMP-2 and TIMP-2 were analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method; the expression of MMP-2 protein was examined by immunohistochemistry. Results: The growth of SGC-7901 cells was inhibited by doxycycline in a time-and dose-dependent manner (P<0.01). Doxycycline decreased the expression of MMP-2 mRNA and MMP-2 protein, but increased the expression of TIMP-2 mRNA dose-dependently (P<0.05). Conclusions: Doxycycline can inhibit the proliferation of SGC-7901, which may be related to its effect of down-regulating MMP-2 and up-regulating TIMP-2.更多还原