Myoglobin as Mimetic Enzyme and Its Analytical Application in Determination of H₂O₂

【Abstract】 The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of H₂O₂ with o-phenylenediamine as substrate. The absorbance obtained at 40 ℃ was found to be proportional to the concentration of H₂O₂ in the range of 2.0×10 -5 -2.0×10 -7 mol/L, with a correlation coefficient of 0.999 1. The proposed method is simple and highly sensitive to a detection limit of 2.7×10 -8 mol/L. The relative standard deviations were within 5% for the determination of different concentrations of H₂O₂. Satisfied results were obtained in the determination of H₂O₂ in rainwater by this method, and the recoveries were at the range of 97.2%-105.4%.更多还原