【摘要】 2-酮戊二酸是碳-氮代谢的重要调控因子,在培养液中添加2-酮戊二酸可以促进水稻幼苗根部碳-氮代谢酶的活性.为了确定2-酮戊二酸是否与这些酶之间存在直接作用(体内实验),取正常培养的水稻幼苗材料,在上述酶的测活体系中分别添加2-酮戊二酸,并进行酶活性测定(体外实验).实验结果表明:2-酮戊二酸可以促进水稻幼苗根组织提取液中GS,HXK的活性,而对GOGAT,ICDH没有明显的影响.上述结果说明2-酮戊二酸作为一种效应物可能与GS、HXK结合,从而在体内、体外都促进了它们的活性.更多还原

【Abstract】 In carbon and nitrogen metabolisms of higher plants,2-oxoglutarate is an important regulation effector.Some experiments have already showed that 2-oxoglutarate could promote enzymes of carbon and nitrogen metabolisms in roots of rice seedlings roots(experiment in vivo),for example,HXK、GS、GOGAT and ICDH.To conform whether direct interaction between 2-oxoglutarate and these enzymes,we made enzyme extracts from rice seedling roots in normal culture condition(free 2-oxoglutarate),and the different concentrations of 2-oxoglutarate were added to the reaction systems of the above enzymes,and determined their activities(experiment in vitro),respectively.The results showed that the activities of GS,HXK were promoted by 2-oxoglutarate,but GOGAT and ICDH were not affected.This suggested that 2-oxoglutarate,which was neither their substrate nor their product,maybe as an effector of GS and HXK to promote their activities both in vivo and in vitro.更多还原