【摘要】 确定了99Tcm-环丙沙星(ciprofloxacin,CPF)的最佳标记条件,在此基础上制备了环丙沙星冻干品药盒,并初步观察了99Tcm-CPF在炎症模型小鼠体内的分布。结果显示最佳标记条件为:盐酸环丙沙星的用量大于4 mg;SnCl2.2H2O的用量大于100μg,体系pH为3.0~3.5,反应时间约10 min。最佳标记条件下制备的99Tcm-CPF放化纯度>95%,室温放置6 h,其放化纯度无明显变化;药盒分别在0~8℃冷藏及-18℃冷冻保存3个月后再标记,标记物的放化纯度仍>95%;小鼠炎症模型实验结果显示,注射后4 h脓肿与肌肉放射性摄取比为4.30±0.31。更多还原

【Abstract】 Ciprofloxacin（CPF） made in China is labeled with ⁹⁹Tc^m and the factors which affects the labeling efficiency are determined.Accordingly,a new CPF cold kit is developed and the biodistribution of ⁹⁹Tc^m-CPF in infected animal is evaluated.Under the optimal conditions of pH 3.0～3.5,CPF contents above 4 mg,SnCl₂·2H₂O contents over 100 μg,10 minutes after injection of ⁹⁹Tc^mO₄^-,the radiochemical purity of ⁹⁹Tc^m-CPF is above 95% and remained >95% at 6 h after preparation at room temperature.The kit is stored at 0⁸ ℃ or-18 ℃ in refrigerator,the radiochemical purity of ⁹⁹Tc^m-CPF keeps up with over 95% after 3 months.Mice with infectious lesions injected with ⁹⁹Tc^m-CPF show a mean abscess-to-muscle ratio of 4.30±0.31 at 4 h post injection.更多还原