【摘要】 目的通过光敏剂介导的光氧化反应处理牛颈静脉带瓣管道,观察其理化与抗钙化性能。方法新鲜牛颈静脉带瓣管道24根,随机分为3组：光氧化反处理组(Ⅰ组)；戊二醛处理组(Ⅱ组)；新鲜对照组(Ⅲ组),不作固定处理。3组分别测组织含水量、最大应力值、组织蛋白含量；前两组植入活体1月后测组织含钙量。结果与Ⅲ组相比,Ⅰ组和Ⅱ组的管道组织蛋白含量都明显减少(P＜0．05),最大应力值均明显提高；组织含水量方面,Ⅰ组与Ⅲ组无差异,Ⅱ组明显减低(P＜0．05)；但钙化程度上,Ⅰ组显著低于Ⅱ组(P＜0．001)。结论经光氧化反应处理的牛颈静脉带瓣管道特性更接近于新鲜组织,但又同戊二醛处理的管道一样,具有组织稳定性,且钙化程度低,可望成为一种好的临床生物材料。更多还原

【Abstract】 Objective:To observe the physiochemical properties and calcification resistance of the bovine jugular con- duits with valves stabilized by dye-mediated photo-oxidation Methods:Twenty-four bovine jugular conduits with valves were divided into 3 groups and treated with dye-mediated photo-oxidation (GroupⅠ),glutaraldehyde (GroupⅡ) and untreated group (GroupⅢ) respectively.Tissue water content,breaching strength and tissue protein content were studied.Pieces ofⅠandⅡgroups were implanted in back of SD rats.30 days later,calcium concent analysis was carried out.Results:There was no difference in tissue water content between GroupⅠandⅢ,but there was significant difference between GroupⅠandⅡ.the breaching strength of GroupⅠwas higher than that of GroupⅢ(P＜0.05),but lower than that of GroupⅡ(P＜0.05).A decrease in tissue protein content was found in GroupⅠandⅡ.The tissue calcium content of GroupⅠwas obvi- ously lower than that of GroupⅡ(P＜0.001).Conclusions:The dye -mediated photo-oxidation can effectively preserve the tissue stability and reduce the calcification.更多还原