【摘要】 目的研究Survivin基因在食管癌组织中的表达及其与临床病理指标的关系,并探讨其在食管癌发生、发展中的作用。方法采用逆转录多聚酶链反应技术检测Survivin基因在50例食管癌组织及其相应癌旁组织和30例正常食管组织中的表达情况并分析它与食管癌临床病理指标的关系。结果50例食管癌组织中Survivin基因的总阳性率为72%,相应癌旁组织中Survivin基因的总阳性率为2%,而30例正常食管组织无一例阳性。食管癌组织中Survivin基因的阳性表达与食管癌患者的年龄、性别无关。Ⅰ、Ⅱ、Ⅲ、Ⅳ期食管癌的阳性表达率分别为25%、50%、89.4%、92.3%,随着临床分期越晚,Survivin基因的阳性表达率呈增高趋势。高分化鳞癌、中分化鳞癌、低分化鳞癌的食管癌组织中Survivin基因的阳性表达率分别为68.4%、72.7%、77.8%,呈逐渐增高趋势。有淋巴结转移的食管癌组织中Survivin基因的阳性表达率为93.1%,无淋巴结转移的食管癌组织中Survivin基因的阳性表达率为42.8%。结论Survivin基因在正常食管组织不表达,在食管癌旁组织低表达,而在食管癌组织高表达,说明Survivin基因的异常表达而引起的细胞凋亡抑制在食管癌的发生、发展过程中起了一定的作用。Sur-vivin基因的表达可以作为预后不良的重要指标。更多还原

【Abstract】 Objective: To study the expression of Survivin gene in carcinoma of the esophagus and assay for clinical pathologyical significance.Methods: The expression of Survivin gene was detected in 30 cases of normal tissues of the esophagus,and 50 cases of esophageal carcinoma tissues and tumor-adjacent normal tissues by Reverse Transcription Polymerase Chain Reaction and its relationship with the clinicopathological parameters of carcinoma of the esophagus was disclosed.Results: The overall positive rate of Survivin gene expression in the 50 cases of esophageal carcinoma tissues was 72%,and in tumor-adjacent normal tissues 2%.No Survivin was found in 30 cases of normal tissues of the esophagus.The positive rate of Survivin gene expression had no relation to gender and age.The positive rates of Survivin gene expression in stage Ⅰ,Ⅱ,Ⅲ,Ⅳ esophageal carcinoma were 25%,50%,89.4%,92.3% respectively,and the expression of Survivin gene was higher in later clinical stages of esophageal carcinoma.The positive rates of Survivin gene expression in highly differentiated,neutral differentiated and lowly differentiated esophageal carcinoma were 68.4%,72.7%,77.8%,respectively,and showed a trend of increase.The positive rate of Survivin gene expression in carcinoma of the esophagus with and without local lympth node metastasis was 93.1% versus 42.8%.Conclusion: In this study,we find that Survivin gene expression is significantly increased in esophageal carcinoma tissues and gently increased in tumor-adjacent normal tissues while it was undetectable in the benign tissues of the esophagus,which means that the cell apoptosis resulted from the abnormal expression of Survivin gene affects the occurrence of esophageal carcinoma.The expression of Survivin gene is an important marker of dysprognosis of tumor.更多还原