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维生素E对烹饪油烟凝集物氧化应激作用的影响

Influence of Vitamin E on oxidative stress induced by cooking oil fume on human peripheral lymphocytes.

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【作者】 宗传龙周白云郭淼赵静

【Author】 ZONG Chuan-long~1,ZHOU Bai-yun~2,GUO Miao~1,ZHAO Jing~1(1.Department of Basic medicine,Taishan medical college,Taian 271000,China;2.Department of Birth control,Taishan medical college,Taian 271000,China)

【机构】 泰山医学院基础医学部泰山医学院计划生育系泰山医学院基础医学部 山东泰安271000山东泰安271000

【摘要】 目的探讨维生素E对烹饪油烟凝集物(condensates of cooking oil fume,COF)处理后的人外周血淋巴细胞产生氧化应激的影响。方法用烹饪油烟凝集物作用于经维生素E预先处理后的人外周血淋巴细胞,然后用2’,7’-二乙酰二氯荧光素(ECFH-DA)检测细胞内活性氧自由基(ROS)的水平,另外,对细胞内超氧化物歧化酶(SOD)活性和脂质过氧化物(LPO)水平也同时检测。结果1.67μg/ml COF作用下人外周血淋巴细胞内ROS和LPO水平增高,SOD活性降低。预先用0.91μl/ml VE处理后,与相应未经VE处理组相比,细胞内ROS和LPO水平降低,SOD活性增强,差异有显著性(P<0.05)。结论0.91μl/mlVE能保护人外周血淋巴细胞免受COF中的氧自由基的攻击,减轻COF导致的氧化应激。

【Abstract】 Objective:To approach how Vitamin E(VE) influences oxidative stress on human peripheral lymphocytes induced by cooking oil fume(COF).Methods:1.67μg/ml COF was mixed with human peripheral lymphocytes pretreated with or without VE at least four hours,then reactive oxygen species(ROS) in the lymphocytes was detected by 2’,7’-dichlorofluorescein diacetate(DCFH-DA).In the meantime lipid peroxide(LPO) and superoxide dismutase(SOD) activity were detected as well.Results:After pre-incubating 0.91μl/ml VE with COF-treated cells,ROS and LPO level in lymphocytes increased,whereas SOD activity decreased significantly in contrast to lymphocytes treated with COF only.Conclusion:In human peripheral lymphocytes,oxidative stress caused by COF could be prevented effectively by 0.91μl/ml VE.

  • 【文献出处】 泰山医学院学报 ,Journal of Taishan Medical College , 编辑部邮箱 ,2006年02期
  • 【分类号】R96
  • 【下载频次】58
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