【摘要】 目的探讨卵巢子宫内膜异位症组织中PTEN基因mRNA及蛋白的表达及其临床意义。方法应用半定量逆转录聚合酶链反应(RT-PCR)检测卵巢子宫内膜异位症组织和正常卵巢组织中PTEN基因外显子1—9和5—9的mRNA表达水平,免疫组织化学方法检测其蛋白表达,同时结合病人的临床资料进行分析。结果卵巢子宫内膜异位症组织中PTEN外显子1—9、5—9 mRNA相对表达水平高于正常卵巢组织,差异有显著性(P<0.01,P<0.05)。卵巢子宫内膜异位症组织中PTEN mRNA表达水平与病人年龄、囊肿大小无关(P>0.05)。与临床分期有关(P<0.05)。卵巢子宫内膜异位症组织中Ⅰ~Ⅱ期有2例细胞核PTEN蛋白呈中等染色表达,与正常卵巢组织中PTEN蛋白的表达比较差异无显著性(P>0.05)。结论PTEN mRNA及蛋白表达水平的异常可能是卵巢子宫内膜异位症发生的早期事件。更多还原

【Abstract】 Objective: To investigate PTEN mRNA and protein expressions in the ovarian endometrioma and its clinical significance.Methods: The expressions of PTEN mRNA and protein were examined by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry respectively in the ovarian endometrioma and normal ovarian tissues.The clinical characteristics were compared and clinical significance was analyzed.Results: The mRNA expressions of exons 1-9,5-9 in PTEN gene in ovarian endometrioma tissues were significantly higher than those in normal ovarian tissues(P<0.01,P<0.05).The expression level of PTEN mRNA in ovarian endometriosis was related to staging(P<0.01),but not to the patient age or the size of cyst(P>0.05).There was no significant difference in the expression of PTEN protein between ovarian endometrioma and normal ovarian tissues(P>0.05).Conclusion: The abnormal expressions of PTEN mRNA and protein may represent an early event in the genesis of ovarian endometrioma.更多还原