【摘要】 建立反相高效液相色谱法(RPHPLC)测定三甲基氢醌含量的方法。采用CAPCELLPAKMGⅡC18为分析柱(4.6mm×250mm,5μm),水甲醇(45∶55)为流动相,流速为1.0ml/min,检测波长为280nm,柱温为25℃。三甲基氢醌在0.10～0.26mg/ml范围内浓度与峰面积比呈良好的线性关系(R=0.9999)。更多还原

【Abstract】 A reversed phase high performance liquid chromatography （RP-HPLC） method was established for the determination of 2,3,5-trimethylhydroquinone. The column was CAPCELL PAK MGⅡ C18（4.6mm×250mm,5μm）, the mobile phase consisted of methanol and water （w（MeOH）/w（H₂O）=55∶45）, the flow rate was 1.0 ml/min, the detection was done at λ=280nm, and the column temperature was 25℃. The line range for 2,3,5-trimethylhydroquinone was 0.10～0.26mg/ml （R=0.9999）.更多还原