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苦茶Camellia assamica var. kucha茶多酚的HPLC-DAD/MS分析

HPLC-DAD/MS Analysis of Tea Polyphenols in Camellia assamica var. kucha

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【作者】 王冬梅杜丽丽卢嘉丽石祥刚宋晓虹杨得坡叶创兴

【Author】 WANG Dong-mei~1,DU Li-li~2,LU Jia-li~1,SHI Xiang-gang~2,SONG Xiao-hong~2,YANG De-po~1,YE Chuang-xing~(2*)(1.School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510080,China;2.School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)

【机构】 中山大学药学院中山大学生命科学学院中山大学生命科学学院 广州510080广州510275广州510080

【摘要】 苦茶是一种特殊的茶组植物,它以1,3,7,9-四甲基尿酸为主要的嘌呤生物碱。本文采用高效液相色谱-二极管阵列检测/质谱联用(HPLC-DAD/MS)的方法对苦茶水提液中的茶多酚和嘌呤生物碱进行了定性分析,检测到3种嘌呤生物碱、7种儿茶素类化合物和2种非儿茶素类茶多酚(没食子酰奎宁酸酯和咖啡酰奎宁酸酯)。同时与传统绿茶在儿茶素类化合物的组成和含量上进行了比较,结果显示,苦茶中总儿茶素含量(13.82%)远高于传统绿茶(7.37%),但各儿茶素类化合物的相对组成比在两种茶中极其相近,均以酯型儿茶素为主。

【Abstract】 Kucha(Camellia assamica var.kucha) is a special wild tea resource,containing 1,3,7,9-tetramethyluric acid(theacrine) as the predominant purine alkaloid in the leaves.Using HPLC-DAD/MS analysis,the qualitative and primarily quantitative studies of tea polyphenols in Kucha water extract were carried out.As the results,besides of 3 purine alkaloids,there were 7 catechins and 2 non-catechin type of polyphenols(galloylquinic acid and caffeoylquinic acid)identified or tentatively identified.Compared with traditional green tea(C.sinenesis),total content of catechins in Kucha was much higher.However,the relative compositions of catechins were quite similar in both of Kucha and traditional green tea,possessing ester type of catechins as the major polyphenols.

【关键词】 苦茶茶多酚儿茶素液质联用
【Key words】 Kuchatea polyphenolscatechinsHPLC-DAD/MS
【基金】 中山大学张宏达科学研究基金项目(ZHD2004001)
  • 【文献出处】 天然产物研究与开发 ,Natural Product Research and Development , 编辑部邮箱 ,2006年06期
  • 【分类号】R284
  • 【被引频次】17
  • 【下载频次】604
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