【摘要】 目的检测核因子κB(nuclear factor-κB,NF-κB)在分泌性中耳炎(secretory otitis media,SOM)动物模型鼓室黏膜中的表达,探讨NF-κB活化在SOM发病机制中的作用。方法应用灭活肺炎链球菌注入豚鼠中耳腔,制作SOM动物模型,采用免疫组织化学的方法检测致病鼠鼓室黏膜上皮在注入细菌后6h、1d、3d、7d及14d后NF-κBp65、TNF-α和IL-1β的表达及其关系。结果SOM动物模型的鼓室黏膜上皮中NF-κBp65在术后6h就有表达,24小时表达最强,随后表达明显下降,TNF-α和IL-1β的表达也出现相应波动。结论灭活肺炎链球菌致SOM的早期,NF-κB即被激活,在鼓室黏膜中出现过度表达,并与SOM发病中的重要致炎因子TNF-α和IL-1β的产生相关,提示NF-κB与SOM发病机制密切相关。更多还原

【Abstract】 Objective To explore the expression of nuclear factor-κB (NF-κB) in the mucosa of guinea pigs with secretory otitis media(SOM) and the role of NF-κB in the development of SOM.Methods SOM was produced by injecting deactivated streptococcus pneumoniae into the typmpanic cavity of guinea pigs. The expression of NF-κBp65, TNFα, and IL1β was examined by means of immunohistochemistry 6 h,1D,3D,7D,and 14D after injection.Results NF-κBp65 expression could be detected in the middle ear mucosa as early as 6 h after injection,and reached maximum at 24 h. The expression pattern of TNFα and IL1β was similar to that of NF-κB.Conclusion NF-κB is activated in the early stage of SOM induced by deactivated streptococcus pneumoniae. Moreover, NF-κB can induce TNFα and IL1β expression. It indicates that NF-κB may be a important role in the pathogenesis of SOM.更多还原