【摘要】 目的探讨耳蜗侧壁α2Na,K-ATPase通道在听觉功能和年龄相关性听力减退中的作用。方法利用半拷贝基因的α2Na,K-ATPase+/-杂合子小鼠作为试验平台,采用听性脑干反应(ABR)和耳蜗内电位(endoco-chlear potential,EP)检测方法,对α2Na,K-ATPase+/-杂合子小鼠和α2Na,K-ATPase+/+野生型小鼠进行ABR和EP检测,并对各个鼠龄段的小鼠进行ABR检测。结果α2Na,K-ATPase+/-杂合子小鼠的听力和EP值均低于α2Na,K-ATPase+/+野生型鼠;α2Na,K-ATPase+/-杂合子小鼠的听力随鼠龄增长而逐渐下降,高龄期小鼠的ABR阈值与其低龄时相比显著性升高(P<0.05)。结论耳蜗侧壁的α2Na,K-ATPase通道对听觉功能具有重要作用;携带半拷贝基因的αNa,K-ATPase+/-杂合子小鼠表现出轻度年龄相关性听力减退。更多还原

【Abstract】 Objective Using α₂ Na,K-ATPase mouse model to assess the role of α₂ Na,K-ATPase in auditory function and the relationship with age-related hearing loss（AHL）.Methods Auditory function of α₂ Na,K-ATPase +/- mice and wild type mice were detected regularly by auditory brainstem response（ABR） and endocochlear potential（EP）.Results The mean value for ABR thresholds in response to stimulus was elevated in aged α₂ Na,K-ATPase +/- mice, relative to that in younger α₂ Na,K-ATPase +/+ mice significantly （P<0.05）.The EP value of α₂ Na,K-ATPase +/- mice was significantly decreased than that observed in α₂ Na,K-ATPase +/+ mice（P<0.05）. α₂ Na,K-ATPase +/- mice showed a slightly progression of age-related hearing loss.Conclusion The α₂ Na,K-ATPase in cochlear lateral wall is important to cochlear auditory function. α₂ Na,K-ATPase gene maybe be responsible for the pathogenesis of Presbycusis. Mice that expressed only one copy of α₂ Na,K-ATPase may develop to AHL.更多还原