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复方免疫增强剂中三种多糖的分离纯化初步研究

Study on Separation and Purification of Polysaccharides in GCI

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【作者】 戴永强刘红廉宜君谷新利李炳奇

【Author】 Dai Yongqiang~1 Liu Hong~2 Lian Yijun~2 Gu Xinli~3 Li Bingqi~(2*)(1 College of Life Science,Shihezi University,Shihezi,Xinjiang 832003)(2 School of Chemistry and Chemical Engineering,Shihezi University,Shihezi,Xinjiang 832003)(3 College of Animal Science and Technology,Shihezi University,Shihezi, Xinjiang 832003)

【机构】 石河子大学生命科学学院石河子大学化学化工学院石河子大学动物科技学院石河子大学化学化工学院 新疆石河子832003新疆石河子832003

【摘要】 采用超声辅助提取法提取复方免疫增强剂中的多糖,以Sevag法除蛋白后分级醇沉得粗多糖PS1、PS2和PS3。粗多糖PS1、PS2和PS3上离子交换柱层析分离纯化,以0-2.0MnaC l溶液进行梯度洗脱得PS1A、PS2A和PS3A多糖组分,再经过Sephadex G-200凝胶柱层析分离得到三个主要多糖组分PS1A-Ⅰ、PS2A-Ⅱ和PS3A-Ⅲ。利用凝胶层析、纸层析及紫外分光光度法进行纯度鉴定,结果表明PS1A-Ⅰ、PS2A-Ⅱ和PS3A-Ⅲ为均一多糖。

【Abstract】 In this study,we extracted the polysaccharides in the compound immunoenhancer,adopting ultrasonic-assisted extraction.We got three rude polysaccharides: PS1,PS2 and PS3 using ethanol depositing.Then PS1,PS2 and PS3 were isolated by DEAE-Cellulose ion-exchange chromatography and Spandex G-200 gel column chromatography,and three main polysaccharides PS1A-Ⅰ,PS2A-Ⅱand PS3A-Ⅲ were obtained.It’s proved that these three polysaccharides were homogeneous by gel chromatography,paper chromatography and UV-Spectrophotometers.

【基金】 新疆维吾尔自治区自然科学基金(04HJ05)
  • 【文献出处】 塔里木大学学报 ,Journal of Tarim University , 编辑部邮箱 ,2006年02期
  • 【分类号】S853.7
  • 【被引频次】3
  • 【下载频次】147
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