【摘要】 目的:探讨粒细胞集落刺激因子(G-CSF)对心肌梗死大鼠心肌T淋巴细胞亚群的影响。方法:29只大鼠随机分为干细胞动员组(GAMI,n=9)、心肌梗死对照组(AMI,n=10)和假手术组(SO,n=10)。GAMI组和AMI组结扎左冠状动脉造成心肌梗死,SO组行相似的手术操作但不结扎冠状动脉。GAMI组给予rhG-CSF(50μg·kg-1·d-1),皮下注射,共7d。AMI组及SO组则每日皮下注射同等体积的生理盐水,共7d。心肌梗死后4周,通过免疫组化染色检测心肌CD3+、CD4+和CD8+细胞计数并计算CD4+/CD8+比率。结果:AMI组大鼠有3只分别于术后第3、7、8天死亡,GAMI组与SO组大鼠至观察结束无一只死亡。与AMI组相比较,GAMI组CD3+和CD8+细胞计数降低(均P<0.01),CD4+细胞计数无明显变化(P>0.05),CD4+/CD8+比率升高(P<0.01)。结论:G-CSF可降低心肌梗死后大鼠心肌CD3+和CD8+T淋巴细胞计数、升高CD4+/CD8+比率,提示G-CSF可减轻心肌梗死后的免疫损伤。更多还原

【Abstract】 Objective: To investigate T lymphocyte subsets changes affected by treatment of G-CSF in acute myocardial infarction rats. Methods: Twenty-nine rats were randomly divided into there groups, GAMI(n = 9),AMI(n = 10) and SO(n = 10). The left coronary artery was ligated in GAMI and AMI groups result in AMI and sham-operation was performed in SO group. Rats of GAMI group were subcutaneously injected with rhG-CSF(50 μg·kg-1·d-1) for seven days. Saline was given by the same manner in the sham-operated and the AMI group for seven days. After four weeks of infarction, the number of CD3+,CD4+ and CD8+ cells per field of a microscope was detected and CD4+/CD8+ ratio was obtained through the stain of immunohistochemistry. Results: Three rats in the AMI group died on the third,seventh and eighth day after myocardial infarction. No rats died in GAMI and AMI groups. A significant decrease in the number of CD3+ and CD8+ cells per field combined with a significant increase in CD4+/CD8+ ratio were documented in the GAMI group (P < 0.01, P < 0.01) respectively and there was no statistic significance in CD4+ cells (P > 0.05)compared with the AMI group. Conclusion: The treatment of G-CSF can reduce the number of CD3+, and CD8+ per field ,enhance CD4+/CD8+ ratio in rats with myocardial infarction. These findings suggest that G-CSF may play an important role in relieving the immune injury induced by T lymphocyte in acute myocardial infarction rats.更多还原