【摘要】 目的探讨转化生长因子-β1(TGF-β1)在肺癌患者CD4+CD25+调节性T细胞比例增高中发挥的作用。方法采用酶消化法培养原代肺癌细胞,将培养的肺癌细胞及肺癌细胞培养上清液分别与自身来源的外周血单个核细胞共培养,不同浓度(2、51、0 ng/ml)的TGF-β1进行干预后,检测各组CD4+CD25+调节性T细胞标志物FOXP3 mRNA的表达。结果肺癌细胞与外周血单个核细胞共培养组FOXP3 mRNA的表达呈阳性,且随着TGF-β1干预浓度的增加,FOXP3 mRNA的表达强度逐渐增高,两者呈正相关(r=0.663,P<0.05);外周血单个核细胞经肺癌细胞培养上清液孵育后亦有FOXP3 mRNA的表达,TGF-β1干预后,FOXP3 mRNA的表达虽有上调但与干预前相比,差异无显著性意义(P>0.05)。结论TGF-β1能上调外周血单个核细胞中FOXP3的表达,即增加CD4+CD25+调节性T细胞的比例,从而参与肺癌免疫逃逸机制。更多还原

【Abstract】 Objective To explore the role of TGF-β1 in up-regulating the expression of FOXP3 gene in CD4~+CD25~+ regulatory T cells of lung cancer patients.Methods The freshly resected lung cancer specimens were treated with the method of enzymatic digestion.Cancer cells were isolated and cultivated.The cultured lung cancer cells(group A) and the culture supernatant(group B) of lung cancer cells were separately co-cultured with peripheral blood mononuclear cells(PBMCs).After the CD4~+CD25~+ regulatory T cells were stimulated with different levels of TGF-β1(10,5 and 2 ng/ml) respectively,the expression of FOXP3 mRNA in each group was detected to illustrate the correlation between FOXP3 and TGF-β1.Results In group A,the expression of FOXP3 mRNA was positive,and with the increase of TGF-β1 concentration,the expression intensity of FOXP3 mRNA was gradually increased(r=0.663,P<0.05).In group B,the expression of FOXB3 mRNA in the supernatant originated from primary cultured lung cancer cells co-caltured with PBMCs had no obviousl change with the increase of TGF-β1 concentration(P>0.05).Conclusion TGF-β1 could up-regulate the expression of FOXP3 mRNA,which might play a key role in the increased CD4~+CD25~+ regulatory T cells of lung cancer patients.更多还原