【摘要】 目的从抗氧化性及改善三磷酸腺苷酶(ATPase)活性两方面,研究异莲心碱(IL)对实验性前脑缺血/再灌注(I/R)损伤保护作用的机制。方法采用双侧颈总动脉栓塞(BCCAO)合并低血压制备小鼠前脑I/R损伤模型,实验分为假手术组,I/R损伤组,尼莫地平(Nim)阳性对照组和IL治疗组。采用生物化学法分别测定脑组织和脑细胞线粒体中丙二醛(MDA)、活性氧(ROS)、总过氧化物歧化酶(T-SOD)及ATPase的变化。结果①与假手术组比较,I/R损伤组脑组织和脑细胞线粒体中MDA、ROS含量显著升高,而T-SOD、Ca2+-Mg2+-ATPase和Ca2+-ATPase活性明显降低(均P<0.05)。②IL治疗组脑组织和脑细胞线粒体中MDA、ROS含量显著降低,而T-SOD活力改善,Ca2+-Mg2+-ATPase和Ca2+-ATPase活性增加,与I/R组相应指标比较,差异均有显著性意义(均P<0.05),与Nim组相应指标间的差异无显著性意义(均P>0.05)。结论IL对实验性前脑I/R损伤的保护作用机制可能与减少脑组织和脑细胞线粒体中过氧化物和氧自由基含量,增高SOD活性及改善ATPase活性有关。更多还原

【Abstract】 Objective To investigate the protective effects and mechanism of isoliensinine(IL) on procerebrum ischemia/reperfusion injury in mice.Methods Mice were subjected to procerebrum ischemia/reperfusion injury induced by bilateral common carotid artery occlusion(BCCAO) and hypotension.The brains were removed and immediately homogenized,from which mitochondria were extracted,and the contents of MDA and ROS,the activities of SOD and ATPase were detected in brain tissue and mitochondria respectively.Results IL(5,10 and 20 mg/kg) or Nim(1 mg/kg) could strikingly decrease the contents of MDA and ROS of brain tissue,while significantly enhance the activities of SOD and ATPase,compared with ischemia/reperfusion group.Moreover,the same influence on MDA,ROS,SOD and ATPase of mitochondria of brain tissue as that on those of brain tissue could be observed,too.Conclusion IL possesses the protective effects on procerebrum ischemia/reperfusion injury in mice and its mechanism may be related to anti-oxidant action and improvement of the energy metabolism.更多还原