【摘要】 目的研究重楼提取物对肝癌HepG2细胞的毒性作用及其机制。方法应用锥虫蓝拒染法测定不同浓度重楼提取物作用不同时间后对HepG2细胞存活率的影响。通过倒置相差显微镜、透射电镜及苏木精-伊红染色后光镜观察重楼提取物对HepG2细胞形态学的影响。通过碘化丙啶染色法及流式细胞术对重楼提取物是否诱导细胞凋亡进行验证。结果各浓度重楼提取物都对肝癌HepG2细胞有一定的杀伤作用,浓度越高、作用时间越长,其杀伤作用越强;与阳性对照组(FT-207)相比,62.5、125μg/ml重楼提取物对HepG2细胞的杀伤作用较弱(P<0.01,P<0.05),而250、500μg/ml重楼提取物的杀伤作用与其相当(P>0.05),1 000、2 000μg/ml重楼提取物的杀伤作用则明显较高(均P<0.01)。重楼提取物作用后的HepG2细胞呈现变性坏死的形态学改变。流式细胞仪检测结果显示不同浓度重楼组与阴性对照组凋亡率无明显差异(P>0.05)。结论重楼提取物具有细胞毒性作用。重楼提取物可能不是通过诱导细胞凋亡,而是通过导致癌细胞的变性坏死发挥其抗癌作用。更多还原

【Abstract】 Objective To study the cytotoxicity and antitumor mechanism of paridis-extract(PE) on HepG2 cells.(Methods)HepG2 cells were cultured in vitro with PE at different concentrations for different time.The survival rate of the cells in different groups was calculated by trypan blue exclusion method to observe the cytotoxicity of PE on the HepG2 cells.The morphological changes of HepG2 cells caused by PE were observed under phase contrast microscope,optical microscope and transmission electron microscope.The effects of PE on apoptosis were measured by flow cytometry.Results In all the PE groups,PE had cytotoxic effects on HepG2 cells in a dose-and time-dependent manner.As compared with the positive control group(FT-207),the cytotoxicity of 62.5 and 125 μg/ml PE on HepG2 cells was reduced,that of 250 and 500 μg/ml PE was the same(P>0.05),while that of 1 000 and 2 000 μg/ml PE was increased(both P<0.01).After treatment with PE,the changes of degeneration and necrosis occurred in HepG2 cells.The flow cytometry analysis revealed that there was no statistically significant difference in the apoptotic rate between the negative control group and the different concentrations PE groups(P>0.05).Conclusion The PE has in vitro cytotoxicity on HepG2 cells through resulting in the degeneration and necrosis of the tumor cells,but not by inducing apoptosis.更多还原