【摘要】 目的研究血管内皮生长因子(vascular endothelial growth factor,VEGF)和碱性成纤维细胞生长因子(basicfibroblast growth factor,bFGF)在喉癌细胞Hep-2中的表达,及其与喉癌细胞生长、浸润及转移的关系。方法培养Hep-2和正常永生化表皮细胞HaCaT,提取总细胞RNA,应用逆转录聚合酶链反应检测VEGF mRNA和bFGF mRNA在Hep-2和HaCaT细胞中的表达。结果VEGF mRNA在Hep-2细胞中高表达,平均相对积分吸光度值为(0.839±0.063);VEGF mRNA在HaCaT细胞中低表达,平均相对积分吸光度值为(0.305±0.066),差异有极显著性意义(t=14.94,P<0.01)。bFGF mRNA在Hep-2细胞中高表达,平均相对积分吸光度值为(0.792±0.058);bFGF mRNA在HaCaT细胞中低表达,平均相对积分吸光度值为(0.296±0.049),差异有极显著性意义(t=17.54,P<0.01)。结论VEGF及bFGF与喉癌细胞的生长、浸润及转移有密切关系。更多还原

【Abstract】 Objective To study the expression of vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) in human laryngeal carcinoma cells(Hep-2) and its relation with growth,invasion and metastasis of laryngeal cancer cells.Methods The total RNA was isolated from the cultured Hep-2 and human normal keratinocyte line HaCaT.The mRNA expression of VEGF and bFGF in Hep-2 and HaCat cells was detected by using technique of reverse transcriptase polymerase chain reaction.Results Image analysis demonstrated that the expression levels of VEGF mRNA in Hep-2 cells were higher than in HaCaT cells with the corrected integral absorbency being(0.839±0.063) and(0.305±0.066) respectively.There was significant difference between them(t=14.94,P<0.01).The expression levels of bFGF mRAN in Hep-2 cells were higher than in HaCaT cells with the corrected integral absorbency being(0.792±0.058) and(0.296±0.049) respectively.There was significant difference between them(t=17.54,P<0.01).Conclusion VEGF and bFGF was closely related with the growth,invasion and metastasis of laryngeal carcinoma cells.更多还原