【摘要】 采用超临界流体CO2萃取(SFE-CO2)技术提取温莪术Curcuma wenyujinY.H.Chen et C.L ing挥发油,用多种色谱和波谱方法对油中主成分进行分离和结构鉴定。结果分离得到5个环状含氧倍半萜类化合物,分别鉴定为:蓬莪术环二烯(Ⅰ)(furanod iene)、莪术二酮(Ⅱ)(curd ione)、莪术双环烯酮(Ⅲ)(curcum enone)、(4S,5S)大=牛儿酮4,5环氧化物(Ⅳ)((4S,5S)-germ acrone-4,5-epoxide)、莪术烯(Ⅴ)(curzerene);其中蓬莪术环二烯含量约占莪术油30%,莪术双环烯酮系首次从温莪术中得到。并对上述5个化合物进行了THP-1细胞分泌TNFα炎症因子抑制作用的体外活性实验,实验结果表明:蓬莪术环二烯和莪术烯对THP-1细胞分泌TNFα炎症因子有明显的抑制作用。更多还原

【Abstract】 The volatile constituents,extracted from Curcuma wenyujin with supercritical CO₂,were separated with various chromatographic methods,and were identified by the NMR methods.Five major constituents were identified,namely furanodiene,curdione,curcumenone,（4S,5S）-germacrone-4,5-epoxide and curzerene.Curcumenone was obtained from Curcuma wenyujin for the first time.It was found that 30% of the curcuma oil content was of furanodiene.The effect of five sesquiterpenoids on the inhibition of TNF-α,the inflammatory factor excreted by THP-1 cells were examined.Experimental results showed that furanodiene and curzerene have significant inhibition effect on the TNF-α factor.更多还原