【摘要】 报道了用电化学方法、紫外光度法和黏度法对亚甲基蓝(MB)与小牛胸腺DNA(CT DNA)相互作用的研究,求得键合常数为6.0×104L.mol-1,键合位点数为0.7。在DNA修饰电极上氧化态MB比还原态MB的键合常数为Kox/Kred=7。结果表明亚甲基蓝可以与DNA发生强烈的作用,其作用模式为混合模式(主要为静电键合,沟槽键合)。更多还原

【Abstract】 The interaction of methylene blue（MB） with CT DNA was studied by electrochemistry,UV-visible spectroscopy,viscosity measurements.Information such as intrinsic binding costant(k=6.0×10⁴ L·mol^-1),and binding numbers（n=0.7）,ratio(K_ox/K_red=7) of the bind constants for the oxidized and reduced forms of MB were obtained.Interaction mode of MB with DNA were electrostatic attraction and groove-binding.更多还原