【摘要】 建立了高效阴离子交换色谱脉冲安培检测器(HPAEC-PAD)测定碘离子的方法。以62.5 mmol/LNaOH溶液作流动相,选用IonPac AS16型强亲水性阴离子交换分析柱,8 m in可以分离出碘离子,方法的检出限为0.05μg/L(25μL进样,3倍信噪比),标准曲线的线性范围为0.5~2 000μg/L(r=0.999 8)。1.0、2.5和25μg/L的碘离子标准溶液各9次进样的相对标准偏差分别为3.8%、2.6%和2.8%。试验了9种常见阴离子、3种易极化阴离子以及3种电化学活性物质对碘离子测定的影响,结果表明,在所选用的色谱条件下上述各化合物均不干扰碘离子的测定。用所建立的方法测定了盐水、矿泉水、海水、芹菜汁和紫菜浸提液等样品中碘离子含量,加标回收率在83%~114%。该方法具有灵敏度高、干扰小和样品前处理简单等优点。更多还原

【Abstract】 A novel method for the determination of iodide by high performance anion exchange chromatography with pulsed amperometric detector(HPAEC-PAD) was developed.Using 62.5 mmol/L sodium hydroxide solution as mobile phase,iodide can be separated from the matrix within 8 min on high hydrophilic IonPac AS16 column.The calibration graph was linear from 0.5 μg/L to 2 000 μg/L for iodide with a correlation coefficient of 0.999 9.The detection limit was 0.05 μg/L(25 μL injected,signal-to-noise ratio of 3) and the relative standard deviations(RSD) of 1.0,2.5 and 25 μg/L iodide standard solutions were 3.8%,2.6% and 2.8%,respectively(n=9).The effects of 9 common ions,3 polarizable anions and 3 electrochemical active substances on the determination of iodide were studied.The results showed that under optimum chromatographic conditions,the determination of iodide was not interfered by any of them.The established method was applied to the determination of iodide in salty water,mineral water,seawater,celery juice and laver extractants with recoveries ranging from 83% to 114%.更多还原