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银杏叶水溶性多糖的分离、纯化、初步鉴定及活性研究

Studies on Separation,Purifaction of Water Soluble Polysaccharide and Initial Identification and Actiity of Ginkgo biloba L.Leaf

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【作者】 杨静峰张旭梁忠岩张丽霞

【Author】 YANG Jing-feng,ZHANG Xu,LIANG Zhong-yan,ZHANG Li-xia(1.Life Science Department Northeast Normal University.Changchum,130024 China; 2.College of Biology and food Engineering,College of Dalian Light Industry.Dalian,116034 China)

【机构】 东北师范大学生命科学学院大连轻工业学院生物与食品工程学院东北师范大学生命科学学院 吉林长春130024大连116034吉林长春130024

【摘要】 目的:从银杏叶中提取水溶性多糖并进一步纯化,得到均一的多糖PGBL3并研究其组成、性质及其活性。方法:粗多糖经反复冻融沉淀、Sevage法脱蛋白、超滤、柱色谱等方法分离纯化得到级分PGBL3。以比旋光度测定、醋酸纤维薄膜电泳、HPLC等方法检验其均一性,以hela细胞鉴定其抗肿瘤活性。结果:PGBL3为均一组分,GC分析其单糖组成为Gal、Man、Glc、Ara、Rha、GalA,摩尔比为:6.0︰2.4︰1.9︰2.1︰1.9︰1.0。结论:PGBL3为酸性杂多糖,相对分子量为1×105。具有一定抗肿瘤功能。

【Abstract】 objective: A water soluble homogeneous polysaccharide PGBL3 has been extracted from the leaf of Ginkgo Biloba to study it’s composition and charaterization.methods After graded by freezing and purified by the combined deprotionization.methods: of enzyme and sevage,fractionated by ultrafiltration and gel chromatography,it(PGBL3) was homogeneous by tests: Gel filtration on Sephadex G-75,Cellulose acetate pellicle electrophoresis,Determination of specific optical rotation measurement and HPLC analyse.results: PGBL3(Polysaccharide of Ginkgo Biloba Leaf)obtained by gradation and purifaction contain Gal Man Glc Ara Rha GalA,in molar ratio of 6.0∶2.4∶1.9∶2.1∶1.9∶1.0,The anticancer experiment showed that the PGBL3 has some activity for the hela but not very notable.cnclusion: The identification of purificaton implied that PGBL3 was uniform on both molecular weight and polarity,furthmore its molecular weight was about 10 thousand by gel filtration,it was acid polysccharide by G.C.analysis and has some anticancer activity.

【关键词】 银杏叶多糖纯化组成
【Key words】 Ginkgo Biloba leavepolysaccharidepurifactioncompositionactivity.
【基金】 国家自然科学基金项目(39870847)
  • 【文献出处】 特产研究 ,Special Wild Economic Animal and Plant Research , 编辑部邮箱 ,2006年04期
  • 【分类号】S567.19
  • 【被引频次】10
  • 【下载频次】450
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