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长白蝮蛇类凝血酶基因大肠杆菌表达研究(英文)
Expression of Ussurin,a Thrombin-like Enzyme from Agkistrodon halys ussuriensis in Escherichia coli
【摘要】 从长白蝮蛇克隆的类凝血酶Ussurin Cdna被克隆进入pET-32α(+)中与5′端与Thx.Tag和6xHis相连的T7启动子下,构建成大肠杆菌表达载体pET-32α(+)-Ussurin;再转化感受态E.coli BL(21)DE3细胞,构成表达工程菌株;于IPTG诱导下表达,在细胞质中产生约占细胞质总蛋白10%的具有生物活性的Ussurin。
【Abstract】 The gene of Ussurin,a thrombin-like enzyme from Agkistrodon halys ussuriensis,was expressed in Escherichia coli using expression system of pET-32α(+).The gene was expressed under T7 promotor with a fusion partner of Thx.Tag and a 6xHis.Tag at its 5′terminal.After induction by IPTG for 4 h,the recombinant enzyme was expressed in the cytoplasm.Expression amount of recombinant ussurin in cytoplasm is about 10% of the total protein in cytoplasm.
【关键词】 长白蝮蛇;
类凝血酶;
表达;
Ussurin;
【Key words】 Thrombin-like Enzyme; ussurin; Agkistrodon halys ussuriensis; expression;
【Key words】 Thrombin-like Enzyme; ussurin; Agkistrodon halys ussuriensis; expression;
【基金】 This work was supported by The Science Foundation of Hainan Province(No·30239)·
- 【文献出处】 蛇志 ,Journal of Snake , 编辑部邮箱 ,2006年04期
- 【分类号】Q786
- 【被引频次】2
- 【下载频次】65