【摘要】 从长白蝮蛇克隆的类凝血酶Ussurin Cdna被克隆进入pET-32α(+)中与5′端与Thx.Tag和6xHis相连的T7启动子下,构建成大肠杆菌表达载体pET-32α(+)-Ussurin;再转化感受态E.coli BL(21)DE3细胞,构成表达工程菌株;于IPTG诱导下表达,在细胞质中产生约占细胞质总蛋白10%的具有生物活性的Ussurin。更多还原

【Abstract】 The gene of Ussurin,a thrombin-like enzyme from Agkistrodon halys ussuriensis,was expressed in Escherichia coli using expression system of pET-32α（+）.The gene was expressed under T₇ promotor with a fusion partner of Thx.Tag and a 6xHis.Tag at its 5′terminal.After induction by IPTG for 4 h,the recombinant enzyme was expressed in the cytoplasm.Expression amount of recombinant ussurin in cytoplasm is about 10% of the total protein in cytoplasm.更多还原