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长白蝮蛇类凝血酶基因大肠杆菌表达研究(英文)

Expression of Ussurin,a Thrombin-like Enzyme from Agkistrodon halys ussuriensis in Escherichia coli

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【作者】 杜道林王锐萍杨国锋符碧吴文芳

【Author】 DU Dao-lin~(1),WANG Rui-ping~(1),YANG Guo-feng~(1),FU Bi~(1),WU Wen-fang~(2)(1.Department of Biology,Hainan Normal University,Haikou,Hainan,571158,China;2.Institute of Applied Ecology,Chinese Academy of Science,Shenyang,Liaoning,110016,China)

【机构】 海南师范大学生物系中国科学院沈阳生态研究所 海南海口571158海南海口571158辽宁沈阳110016

【摘要】 从长白蝮蛇克隆的类凝血酶Ussurin Cdna被克隆进入pET-32α(+)中与5′端与Thx.Tag和6xHis相连的T7启动子下,构建成大肠杆菌表达载体pET-32α(+)-Ussurin;再转化感受态E.coli BL(21)DE3细胞,构成表达工程菌株;于IPTG诱导下表达,在细胞质中产生约占细胞质总蛋白10%的具有生物活性的Ussurin。

【Abstract】 The gene of Ussurin,a thrombin-like enzyme from Agkistrodon halys ussuriensis,was expressed in Escherichia coli using expression system of pET-32α(+).The gene was expressed under T7 promotor with a fusion partner of Thx.Tag and a 6xHis.Tag at its 5′terminal.After induction by IPTG for 4 h,the recombinant enzyme was expressed in the cytoplasm.Expression amount of recombinant ussurin in cytoplasm is about 10% of the total protein in cytoplasm.

【基金】 This work was supported by The Science Foundation of Hainan Province(No·30239)·
  • 【分类号】Q786
  • 【被引频次】2
  • 【下载频次】65
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