【摘要】 目的探讨p16和bcl-2表达产物在宫颈上皮瘤样病变及宫颈癌中表达的意义。方法对正常宫颈鳞状上皮、宫颈上皮内瘤变(CIN)和宫颈癌组织共69例,采用免疫组织化学EliVision法,对宫颈癌变过程中p16和bcl-2蛋白进行研究,将结果进行统计分析。结果p16蛋白在CIN中的表达高于正常宫颈上皮(P<0.01),在宫颈癌中的表达也高于正常宫颈上皮(P<0.01),且高于CIN中的表达(P<0.05);bcl-2蛋白在CIN中的表达高于正常宫颈上皮(P<0.01),在宫颈癌中的表达也高于正常宫颈上皮(P<0.01),但与CIN中表达无差异。p16和bcl-2两种蛋白在CIN和宫颈癌中的表达无明显差异。结论p16和bcl-2蛋白的表达与宫颈癌的发生有关,提示这两种蛋白有可能作为高危人群早期筛查的一种免疫组化指标。更多还原

【Abstract】 objective To investigate the significance of expression of p16 and bcl-2 in cervical intraepithelial neoplasia ang invisive cervical carcinoma. Methods The expression of p16 and bcl-2 proteins of 69 cases of normal cervical tissue cervical intraepithelial neoplasia and cervical carcinoma was detected by Elivision’s immunohistochemical staining and the results were correlate with the results.Results The expression of p16 was significantly higher in CIN than in normal cervixes(P<0.01),It was significanthly higher in cervical carcinomas than in normal cervixes(P<0.01),and higher than in CIN(P<0.05).The expression of bcl-2 was signinfican higher in CIN than in normal cervixes(P<0.01).It was significanthly higher in cervical carcinomas than in normal ctrvixes(P<0.01),but no statistical defference between in cervical carcinomas and in CIN.No statistical difference of expression in cervical carcinomas and CIN was found between p16 and bcl-2 protein. Conclusions The results indicated that the expression of p16 and bcl-2 protein was associated with the carcinogenesis of cervical cancer.It is suggested that p16 and bcl-2 expression may be taken as an effective immunohistochemical makers for early detection.更多还原