【摘要】 植物组培再生体系的建立是进行基因转化的前提.本文对高羊茅的组培再生体系的建立进行了初步研究.(1)研究了不同的种子灭菌处理方式对高羊茅种子萌发率和污染率的影响,结果显示:高羊茅种子最佳灭菌方式是将种子先在无菌水中浸泡1～3 h,然后用70%酒精灭菌30 s,无菌水冲洗5次,再用50%次氯酸钠灭菌20 min,无菌水冲洗6次.采用此灭菌方式,种子萌发率约为80%,而污染率为0%.(2)将高羊茅下胚轴接种在含有不同浓度2,4-D的MS培养基上进行愈伤组织诱导,结果表明:10 mg/L的2,4-D是下胚轴愈伤组织诱导的最佳浓度,过高的2,4-D浓度对愈伤组织的形成有负作用.(3)将高羊茅下胚轴接种在含有10 mg/L 2,4-D的MS、B5、N6培养基上进行愈伤组织诱导,结果表明:3种不同培养基中,MS培养基为最适培养基.更多还原

【Abstract】 The establishment of tissue culture regeneration system for tall fescue is the foundation for its transformation.In this paper,the establishment of tall fescue tissue culture regeneration system was studied.(1)Different ways of seeds treatment were done to investigate their effects on the germination and pollution of seeds,and the results showed that the optimum method for the sterilization of tall fescue seeds is to soak the seeds for 1～3 h in sterile water and 30 s in 70% alcohol,wash them 5 times with sterile water,then sterilize them for 20 min in 50% sodium hypochlorite and wash them 6 times with sterile water.Using this sterilization method,the ratio of germination is 80%,while that of pollution is 0%.(2) The lower plumular axes of tall fescue were inoculated on the MS mediums with different 2,4-D concentration to induce calli,and the results indicated that 10 mg/L 2,4-D was the optimum concentration on calli induction of tall fescue,high concentration of 2,4-D had negative effect on callus induction.(3) The lower plumular axes of tall fescue were inoculated on the MS、B5、N6 mediums with 10 mg/L 2,4-D concentration to induce calli,and the results indicated that MS is the optimal medium in all of the three media tested.更多还原