【摘要】 目的观察青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子Fas/FasL及Bc l-2/Bax表达的影响。方法雄性W istar大鼠60只随机分为6组,空白组、模型组(对照组)、青蒿琥酯I组(高剂量组)、青蒿琥酯Ⅱ组(低剂量组)、青蒿琥酯加甲氨喋呤组及甲氨喋呤组。除空白组外,各实验组用完全弗氏佐剂制备成佐剂性关节炎模型。记录用药干预后各组关节肿胀指数,免疫组化法检测滑膜组织中凋亡相关因子Fas/FasL,Bc l-2及Bax的表达情况。结果各实验组关节肿胀指数明显降低,与对照组比较差异有统计学意义(P<0.01)。滑膜组织中Fas/FasL及Bax被上调,Bc l-2被下调,与对照组比较差异有统计学意义(P<0.05)。结论实验证实了青蒿琥酯有诱导佐剂性关节炎病情缓解的作用,上调滑膜组织中Fas/FasL及Bax,下调Bc l-2的表达而诱导滑膜细胞凋亡可能是其机制之一。更多还原

【Abstract】 ObjectiveTo observe the expression of Fas/FasL and Bcl-2/Bax correlated with apoptosis in synoviocyte of adjuvant arthritis(AA) with artesunate.MethodsSixty wistar rats were randomly divided into six groups:normal group,model control group,artesunate group I(high dose),artesunate group II(low dose),artesunate plus MTX group,MTX group.Each group was modeled to adjuvant arthritis with complete Freund’s.After treated with artesunate or artesunate plus MTX,the arthrosis index(AI),the expression of Fas/FasL,Bcl-2,Bax,which were correlated with apoptosis in arthrosis synovial membrane tissue were examined by immunohisto chemistry(IHC).ResultsCompared with the model control group,the AI in every experiment group decreased significantly(P<0.01).Compared with the model control group,the expression of Fas/FasL,Bax in arthrosis synovial membrane tissue increased significantly(P<0.05),Bcl-2 decreased significantly(P<0.05).ConclusionThe effect of artesunate on the alleviation of adjuvant arthritis(AA) was proved in experiment,inducing apoptosis in synoviocyte was likely one of mechanisms.更多还原