【摘要】 目的研究新疆紫草提取物AEJ-I、AEJ-Ⅱ对小鼠急性四氯化碳(CC l4)性肝损伤的保护作用。方法腹腔注射0.1%CC l4橄榄油溶液制备小鼠急性化学性肝损伤模型,以肝脏系数、脾脏系数,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST),肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的水平和肝脏病理组织学变化为观测指标,观察AEJ-I、AEJ-Ⅱ对小鼠急性CC l4性肝损伤的影响。结果AEJ-I、AEJ-Ⅱ各剂量组均可显著降低小鼠血清ALT,AST活性(P<0.01);AEJ-I高剂量组能使肝组织中SOD、GSH-Px活性升高、MDA含量下降(P<0.05);AEJ-I低剂量组可以升高肝组织GSH-Px活性(P<0.05),而对肝组织其它指标均无明显影响(P>0.05);AEJ-Ⅱ高剂量组能降低MDA含量(P<0.05),而对肝组织其它指标AEJ-Ⅱ各剂量组影响均不明显(P>0.05);AEJ-I、AEJ-Ⅱ均可不同程度地改善肝脏病理组织性损伤。结论AEJ-I、AEJ-Ⅱ对小鼠急性CC l4性肝损伤具有保护作用。更多还原

【Abstract】 Objective To investigate the protective effects of Arnebia euchroma（Royle） Johnston extracts（ab.AEJ-I、AEJ-II） against CCl₄-induced acute liver injury in mice.MethodsThe acute liver injury animal model was established in mice by intraperitoneal injection of 0.1% CCl₄ olive oil.The coefficient of liver and spleen;alanine aminotransferase（ALT） and aspartate amiontransferase（AST） activities in serum;superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） activities and malondialdehyde（MDA） content in liver homogenate were assayed and hepatic pathological changes were observed.ResultsAEJ-I and AEJ-II significantly decreased the activities of serum ALT and AST in mice（P<0.01）;AEJ-I high dose group increased the activity of liver homogenate SOD,GSH-Px and decreased the content of MDA（P<0.05）;AEJ-I low dose group increased the activity of liver homogenate GSH-Px（P<0.05）,but it had no marked effect on other indexes of liver homogenate mentioned above（P>0.05）;AEJ-II high dose group decreased the content of MDA（P<0.05）,but AEJ-II had no marked effect on other indexes of liver homogenate mentioned above（P>0.05）;AEJ-I and AEJ-II improved liver histopathology injuries.ConclusionAEJ-I,AEJ-II showed protective effects on acute liver injury induced by CCl₄ in mice.更多还原