【摘要】 目的对《中国药典》淫羊藿药材的收载品种巫山淫羊藿中2种黄酮类成分柔藿苷(rouhuoside)和淫羊藿苷(icari-in)进行测定,比较这两种成分在不同月份的含量变化。方法采用反相高效液相色谱(RP-HPLC)法,以beckm ancou lter-1,检测波长为270 nm。结果柔藿苷在TM-C18柱(带预柱)为色谱柱,流动相为乙腈-水(25∶75),流速1.0 m l.m in更多还原

【Abstract】 Objective To determine the content of rouhuoside and icariin in Epimedium wushanense,and compare the content variety of these 2 compounds in different growing seasons.MethodsSamples were dealt with by HPLC with beckmancoulter_TM-C₁₈ analytical column.The mobile phase was consisted of acetonitrilewater（25: 75）.The flow rate was 1.0 ml·min^-1.The UV detection was set at 270 nm.ResultsThe linear response range from （0.196 42） ～ 1.964 2μg of rouhuoside（r=（0.999） 12,n=10）;0.193 35 ～ 1.933 5 μg of icariin（r=0.999 00,n=10）.In the whole growing seasons of Epimedium wushanense,the content of icariin is less than 0.5% except for in May,while the content of rouhuoside is more than 1.710%.ConclusionAs far as rouhuoside and icariin to be concerned,the content of rouhuoside is much higher than that of icariin in each month of the growing seasons of Epimedium wushanense,and rouhuoside is the main component in Epimedium wushanense.更多还原