【摘要】 目的建立同时测定大黄药材中的芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的方法。方法采用反相高效液相色谱(RP-HPLC)法对大黄药材中5种蒽醌及其苷的含量进行测定。色谱条件如下,色谱柱:E M erck L i Chrospher100RP-18(4.6 mm×250 mm,5μm),流动相为甲醇-0.5%高氯酸(85∶15),流速0.8 m l/m in,柱温40℃,检测波长为254nm。结果在此色谱条件下,各蒽醌成分达到基线分离,且峰形良好。芦荟大黄素线性回归方程为:Y=-2 410+537 466 1X,r=0.999 4,在0.016~0.160μg范围内呈良好线性关系,其平均回收率为104.8%,RSD=1.65%;大黄酸线性回归方程为Y=2 148+5 440 831X,r=0.999 7,在0.012~0.120μg范围内呈良好线性关系,其平均回收率为104.0%,RSD=2.71%;大黄素线性回归方程为Y=1 653+4 923 143X,r=0.999 8,在0.017~0.166μg范围内呈良好线性关系,其平均回收率为101.4%,RSD=1.86%;大黄酚线性回归方程为Y=5 823+6 496 978X,r=0.999 8,在0.029~0.286μg范围内呈良好线性关系,其平均回收率为103.2%,RSD=1.93%;大黄素甲醚线性回归方程为Y=3 827+3 385 073X,r=0.999 6,在0.008~0.079μg范围内呈良好线性关系,其平均回收率为102.0%,RSD=1.85%。结论分析方法快速、灵敏、准确,所有待测组分峰分离度良好,达到定量检测的要求。更多还原

【Abstract】 Objective To develop an HPLC method for determination of the Rhubarb anthraquinones（Aloe-emodin,Rhein,Emodin,Chrysophanol,Physcion and their conjunctive compounds） in Radix et Rhizoma Rhei.MethodsRhubarb anthraquinones were simultaneously separated and determined on a Merck Chrospher 100RP-18 column（250 mm×4.6 mm,5 μm）.The detective wavelength was 254 nm.The mobile phase consisted of methanol-0.5%HClO₄（85∶15） and the flow rate was 0.8 ml·min^-1.ResultsUnder the chromatographic condition,the five compounds were completely separated and other components had no effect on their determination.Every Rhubarb anthraquinone’s calibration curve,correlation coefficient,linearity range and average recoveries showed respectively as followed: ①Aloe-emodin berberine: Y=-2 410+5 374 661X,r=0.999 4,linearity range（0.016）～0.160 μg,the average recovery 104.8%,RSD=1.65%.② Rhein berberine: Y=2 148+5 440 831X,r=0.999 7,linearity range 0.012～0.120 μg,the average recovery 104.0%,RSD=2.71%.③Emodin berberine: Y=1 653+4 923 143X,r=0.999 8,linearity range 0.017～0.166μg,the average recovery 101.4%,RSD=1.86%.④ Chrysophanol berberine: Y=5 823+6 496 978X,r=0.999 8,linearity range 0.029～0.286 μg,the average recovery 103.2%,RSD=1.93%.⑤ Physcion berberine: Y=3 827+3 385 073X,r=0.999 6,linearity range 0.008～0.079 μg,the average recovery 102.0%,RSD=（1.85）%.ConclusionThe method is simple,accurate,rapid and sensitive.更多还原