【摘要】 目的用气相色谱-质谱联用法分析川芎愈伤组织及悬浮培养物中的化学成分。方法建立川芎愈伤组织及悬浮培养体系,收集愈伤组织及连续三代的悬浮培养物,采用气相色谱-质谱联用法进行川芎愈伤组织及悬浮培养物化学成分的分析。结果川芎愈伤组织及悬浮培养物含有相似的成分,但与原植物中的成分区别很大,原植物中的主要活性成分在愈伤组织及悬浮培养物中也未发现。结论愈伤组织及悬浮培养物的特性与原植物有很大的区别。更多还原

【Abstract】 ObjectiveTo analyze the components in callus and cell suspension cultures ofLigusticum chuanx-iongHort.by gas chromatography-mass spectrometry(GC-MS).MethodsInitiate the callus and cell sus-pension cultures ofLigusticum chuanxiongHort..Collect the callus and three sequent generations cell suspen-sion cultures,and the components in callus and cell suspension cultures were analyzed by gas chromatography-mass spectrometry.ResultsThe callus and cell suspension cultures ofLigusticum chuanxiongHort.containedsimilar constituenents,but the components in callus and cell suspension cultures were quite different from thoseof the plant,and the main bioactive constituentes of the plant were not found in callus and cell suspension cul-tures.ConclusionThe characteristics of callus and cell suspension cultures were different from those of theplant.更多还原