【摘要】 目的建立三七中人参皂苷Rg1,Rb1的含量测定方法,并比较加工炮制对三七不同饮片中人参皂苷Rg1,Rb1含量的影响。方法Zorbax SB-C18分析柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.05%磷酸溶液梯度洗脱:0~7 m in,25∶75;7~10 m in,35∶65,流速为1.1 m l/m in,柱温为室温,检测波长为205 nm。结果人参皂苷Rg1,Rb1回收率分别为99.46%,98.79%,RSD分别为1.87%,2.13%。各样品中,以三七生药粉中人参皂苷Rg1,Rb1含量为高。结论该方法稳定、准确、简便、快速,为三七质量评价提供参考。三七临床以生品打粉入药为宜。更多还原

【Abstract】 ObjectiveTo establish a determination method and compare the effect of different processing methods on the contents of Ginsenoside Rg₁ and Rb₁ from Radix Notoginseng.MethodsChromatographic conditions:Zorbax SB-C₁₈（4.6 mm×250mm,5μm）column,mobile phase:acetonitrile-0.05%H₃PO₄,0⁷ min,25∶75;7¹0 min,35∶65,flow-rate:1.1 ml/min,room temperature,detection wavelength:205 nm.ResultsThe recovery of Ginsenoside Rg₁, Rb₁ was 99.46%（RSD=1.87%）,98.79%（RSD=2.13%）.The content of them of the Radix Notoginseng powder was the highest among all samples.ConclusionThe method is stable,accurate,simple and rapid,the research can provide the reference for evaluating of Radix Notoginseng.It’s better to use Radix Notoginseng in the form of the powder in clinic.更多还原