【摘要】 目的用高效液相色谱法测定萹蓄中金丝桃苷、槲皮苷与木樨草素的含量。方法采用Sh im adzu C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.2%H3PO4溶液(45∶55),流速1.0 m l.m in-1,检测波长350 nm,柱温室温,用外标法定量,同时测定萹蓄中金丝桃苷与槲皮苷的含量;再采用Sh im adzu C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.2%H3PO4溶液(50∶50),流速1.0 m l.m in-1,检测波长254 nm,柱温室温,用外标法定量,测定萹蓄中木樨草素的含量。结果金丝桃苷的线性范围0.50~2.50μg,r=0.999 8,回收率为95.13%,RSD为2.91%;槲皮苷的线性范围0.40~2.00μg,r=0.999 6,回收率为96.49%,RSD为2.77%;木樨草素的线性范围0.0325~0.39μg,r=0.999 9,回收率为95.75%,RSD为2.49%。结论该方法简便,快速,线性关系良好,可作为萹蓄的含量测定。更多还原

【Abstract】 ObjectiveThe purpose is to determine the content of hyperoside,quercitrin and luteolin in polygonum aviculare by HPLC.MethodsThe analysis of hyperoside and quercitrin was carried out on Shimadzu C₁₈column（150 mm×4.6 mm,5 μm）.The mobile phase was methanol-0.2%phosphate（45∶55）.The flow-rate was 1.0 ml·min^-1and wave-length was 350 nm.Temperature was room temperature.The analysis of luteolin was carried out on Shimadzu C₁₈column（150 mm×4.6 mm,5 μm）.The mobile phase was methanol-0.2% phosphate（50∶50）.The flow-rate was 1.0 ml·min^-1and wave-length was 254 nm.Temperature was room temperature.ResultsThe method was simple and had a good linear relationship.The linear range of hyperoside was 0.50².50 μg and gave a correlation（r） of 0.999 8.The recovery was 95.13%.RSD was 2.91%.The linear range of quercitrin was（0.40）².00 μg and gave a correlation（r） of 0.999 6.The recovery was 96.49%.RSD was 2.77%.The linear range of luteolin was（0.40）².00 μg and gave a correlation（r） of 0.999 6.The recovery was 95.75%,RSD 2.49%.ConclusionThe method is easy and fast to determine the content of hyperoside,quercitrin and luteolin from polygonum aviculare.更多还原