【摘要】 通过设计合适的引物,经RT-PCR方法从里氏木霉的总RNA中扩增出Swollenin基因,并将该基因连接到PMD18-T Simple载体,得到该基因的克隆菌株,经酶切并将该基因连接到巴氏毕赤酵母表达载体pPICZαA,得到重组质粒pPICZαA-swo1.将该重组质粒线性化后,通过电转化方法转化毕赤酵母GS115,得到重组菌株P.pastoris-swo1.该菌株的发酵液可使滤纸纤维强度降低,纤维素膨胀及崩解效果明显,表明重构表达的Swollenin可用于木质纤维素的预处理.更多还原

【Abstract】 The cDNA of swollenin gene was amplified from Trichoderma reesei by RT-PCR and doned in the PMD18-T Simple vector.The fragment of swollenin cDNA was then cut from PMD18-T Simple and ligated with the Pichia expression vector pPICZαA by restriction enzyme,resulting in the recombinant plasmid pPICZαA-swo1.The Linearized pPICZαA-swo1 was transformed into Pichia pastoris GS115 through electroporation,generating a recombinant strain which was named P.pastoris-swo1.P.pastoris-swo1 can reduce the tensility of filter paper fibre,and it swell and loosen cellulose obviously.Results indicate that the recombinant swollenin protein can be used in Pretreatment cellulose.更多还原