【摘要】 目的:探讨长期(5年以上)放置含铜宫内节育器(intrauterinedevice,IUD)与宫颈细胞ras、p53基因突变及人乳头瘤病毒(Humanpapillomaviruses,HPV)感染的关系。方法:采集宫颈脱落细胞学液基标本,采用伯塞斯达系统(thebethesdasystem,TBS)诊断标准行宫颈细胞病理学诊断;采用PCR-SSCP技术检测标本中H-ras、p53基因的表达;采用FQ-PCR技术检测肿瘤相关HPVDNA含量。结果:H-ras及p53基因表达在各组中均无改变;含铜IUD组中HPV6/11型阳性率为3.33%,DNA含量为8.80×107copy/ml,对照组中HPV6/11型阳性率为3.70%,DNA含量为6.75×107copy/ml,两组相比差异无统计学意义(P>0.05)。两组HPV16/18型均为阴性。结论:长期放置含铜IUD(带尾丝)对子宫颈无致突变作用,不增加肿瘤相关HPV感染,不增加子宫颈病变的发生率。更多还原

【Abstract】 Objective: To study the relationship between copper-releasing tailed Intrauterine devices(IUD) and ras p53 gene mutations or human papillomavirus(HPV) infection.Methods: PCR-SSCP technique was used to determine the expression of gene H-ras and p53 in cervical scraping ThinPrep samples.The fixed quantity of HPV DNA was detected by fluorescence quantitative-PCR(FQ-PCR) method.Results: There was no positive expres-sion of gene H-ras and p53 in all cervical scraping samples.The positive rate of HPV6/11 was 3.33% and the fixed quantity of HPV6/11 DNA was 8.80 × 107 copy/ml in copper-releasing tailed IUD group.In control group the positive rate and the quantity of HPV6/11 were 3.70% and 6.75×107 copy/ml,respectively.There was no signifi-cant difference between the two groups.HPV16/18 of all samples were negative.Conclusion: The risk of cervix diseases was not increased for women using copper-releasing tailed IUD for long term.更多还原