【摘要】 目的:探讨脂多糖(LPS)诱导的非特异性炎症对大鼠睾丸功能的影响。方法:利用磁分离均相酶联免疫技术、免疫组织化学、原位杂交方法分别检测血清睾酮(T)、黄体生成素(LH)水平以及生精上皮内增殖细胞核抗原(PCNA)、雄激素结合蛋白(ABP)mRNA的表达。结果:与对照组相比实验组血清T升高、ABPmRNA表达增多(P<0.05)以及生精上皮PCNA表达明显减低(P<0.05),而血清LH变化无显著差异(P>0.05)。结论:睾丸炎症时间质细胞、支持细胞功能改变,致使睾丸局部生精微环境发生变化,可能导致生精障碍。更多还原

【Abstract】 Objective: To study the functional changes of rat testis in condition of the undifferential orchitis stimulated by bacterial lipoplysaccharide (LPS) in vivo. Methods: We examined the changes of serum testosterone, luteinizing hormone (LH) and the expression of proliferating cell nuclear antigen (PCNA), ABP mRNA by magnetic enzyme immunoassay, immunohistochemistry staining and in situ hybrydization technique. Results: Compared with the control group, the serum testosterone and the ABP mRNA expression in sertoli cells rised (P<0.05), and the positive expression of PCNA in seminiferous epithelium was significantly lower (P<0.05). But serum LH level did not change significantly (P>0.05). Conclusion: The functions of Leydig cells and Sertoli cells changed under the testes inflammatory condition, which might make testosterone level in testicular microenvironment change and result in male infertility.更多还原