【摘要】 目的:观察苦参碱对肺腺癌A549细胞生长、凋亡及其对CC10mRNA表达的影响。方法:肺腺癌A549细胞进行培养传代,按苦参碱药物作用浓度由低向高分为A、B、C、D、E组及对照组,培养72h后,MTT实验检测苦参碱对肺癌细胞生长抑制作用;Hoechst33258染色法观察苦参碱对A549细胞凋亡形态的影响,AnnexinV-PI双标法检测苦参碱对A549细胞凋亡率的影响,RT-PCR检测CC10mRNA的表达。结果:不同浓度的苦参碱均具有抑制肺癌A549细胞生长及诱导凋亡作用,A、B、C、D、E组及对照组A549细胞的生长抑制率及凋亡率分别为24.7%、5.12%,33.1%、8.36%,44.3%、10.81%,61.7%、17.10%,74.2%、25.67%及6.2%、3.06%,各组差异具有显著性(P<0.05)。各实验组CC10mRNA阳性表达率分别为1.03±0.07、0.87±0.03、0.74±0.04、0.62±0.06、0.49±0.04。结论:苦参碱抑制肺腺癌A549细胞生长及诱导细胞凋亡的作用可能与其促进CC10mRNA表达上调有关。更多还原

【Abstract】 Objective To observe the effects of matrine on the cell growth, ap optosis and CC10 mRNA expression in lung adenocarcinoma A549 cell line. Methods Human lung adenocarcinoma A549 cells were cultured for 72 hours and divided into experimental group A, B, C, D and E based on the concentrations of matrine rang ed from low to high and one control group. The inhibitory action of matrine on t he growth of lung adenocarcinoma A549 cell line was measured by MTT, the effect of matrine on apoptotic morphology was observed by Hoechst33258 fluorochrome sta ining, the apoptotic rate was evaluated by AnnexinV-PI, and the expression of C C10 mRNA was detected by RT-PCR. Results Matrine with different concentrations could inhibit the cell growth and induce apoptosis in lung adenocarcinoma A549 c ell line. The inhibitory rate of cell growth and apoptotic rate in all experimen tal and control groups were 24.7%and 5.12%, 33.1%and 8.36%, 44.3%and 10.81 %, 61.7%and 17.10%, 74.2%and 25.67%, 6.2%and 3.06%, respectively. There w as a significant difference among the experimental groups and the control group( P< 0.05). The positive rates of CC10mRNA expression in five experimental groups were 1.03±0.07, 0.87±0.03, 0.74±0.04, 0.62±0.06 and 0.49±0.04, respectively . Conclusion The effects of matrine on inhibiting the cell growth and inducing a poptosis in A549 lung adenocarcinoma cell line may be associated with the up-re gulation of CC10 mRNA expression.更多还原