【摘要】 目的:探讨C D4+TG F-β,C D8+TG F-β在哮喘发病机制中的作用,了解IFN-γ和糖皮质激素对其的影响。方法:采用Ficoll-H ypague密度梯度离心及贴壁培养法对18例哮喘患者和10例健康对照者外周血单个核细胞进行分离,纯化后,加入地塞米松及IFN-γ培养24h,加入PM A,莫能菌素,艾罗霉素培养4.5h,促使细胞内细胞因子聚集于高尔基体,取培养上清中的淋巴细胞经流式细胞仪检测分析C D4+T、C D8+T淋巴细胞及其细胞内细胞因子TG F-β表达率。结果:C D4+TG F-β、C D8+TG F-β及C D4、C D8细胞内总TG F-β检测结果的比较,哮喘组C D4+TG F-β(15.50±0.15)%、C D8+TG F-β(19.34±0.18)%,与正常对照组(9.57±0.16)%、(13.49±0.26)%比较,显著增高(均P<0.05);加入地塞米松培养的哮喘组C D4+TG F-β,C D8+TG F-β无显著变化(P>0.05),加入IFN-γ培养的哮喘组C D4+TG F-β(7.61±0.09)%、C D8+TG F-β(9.82±0.19)%的变化显著降低(均P<0.05),C D4、CD8细胞内总TG F-β哮喘组(32.42±1.17)%比正常组(20.53±1.02)%显著增加(P<0.05),加地塞米松后无显著变化(P>0.05),加IFN-γ后(16.85±0.14)%,显著降低(P<0.05)。结论:哮喘急性发作期有C D4+TG F-β、C D8+TG F-β升高;地塞米松不能降低哮喘气道重塑;IFN-γ可通过抑制TG F-β的分泌治疗哮喘,降低哮喘气道重塑。更多还原

【Abstract】 Objective To investigate the expression of TGF-βin peripheral blood of bronchial asthma and its variation under dexamethasone or IFN-γ.Methods Peripheral blood mononuclear cells from 18 asthmatic patients and 10 normal donors were isolated by density centrifugation with Ficoll-Hypague and cultivated with or without dexamethasone、IFN-γrespectively for 24 hours. Until the 19.5th hour,PMA、monesin and ionomycin were added into the cultive palate for continuing 4.5 hours.Then,CD4+T、CD8+T and their intracellular cytokine TGF-βwere analysed by flow cytometery.Results The expression of CD4+TGF-β,CD8+TGF-βin asthmatic group[(15.50±0.15)%,(19.34±0.18)%,respectively] were significantly increased compared with those in normal control group[(0.57±0.16)%,(13.49±0.26)%,respectively](P< 0.05,respectively).While in asthmatic group,TGF-βstimulated with dexmethasone in both CD4+T and CD8+T was no significant difference(P >0.05).The frequency of CD4+TGF-β,CD8+TGF-βin asthmatic group stimulated with IFN-γ[(7.61±0.09)%,(9.82±0.19)%,respectively] had significantly decreased compared with those in asthmatic control group. The total TGF-βin asthmatic control group(32.42±1.17)%was significantly higher than normal(20.53±1.02)%(P< 0.05),and had no difference from that in asthmatic group with dexmethasone(P >0.05) ,but significantly lower than that in asthmatic group with IFN-γ(16.85±0.14)%(P< 0.05).Conclusions There are the rise of CD4+TGF-β,CD8+TGF-βin peripheral blood of acute asthmatic patients.The production of TGF-βcannot be suppressed by dexamethasone which cannot alleviate the airway remodeling. IFN-γmay play a therapic role on asthma by reducing the production of TGF-βin lymphocyte, which is thought to be associated to asthmatic airway remodeling.更多还原