【摘要】 目的了解本地区泌尿生殖道支原体的感染状况,指导临床药物治疗方法用实时荧光定量PCR检测解脲支原体(uu),同时采用支原体培养药敏试剂盒进行Uu和人型支原体(Mh)培养及药敏试验。结果1 298例标本用FQ-PCR共检出Uu 670例,检出率为51.6%。培养法共检出Uu 487例,Mh13例,Uu合并Mh 42例,,两法检出Uu符合率为78.9%。药敏结果示单一Uu感染强力霉素、美满霉素的敏感率较高,分别为81.3%、80.6%。单一Mh感染者,强力霉素、交沙霉素敏感率较高,分别为84.6%、76.9%。而Uu合并Mh感染者对多种抗生素均有较高的耐药率。结论邵阳地区泌尿生殖道支原体检出率较高。FQ-PCR与培养法联合对支原体的诊疗有实际意义。强力霉素可作为本地区单一支原体感染的一线药物,而混合感染应多药联合。更多还原

【Abstract】 Objective To study the status of genitourinary tract mycoplasma infection in our area and to guide the clinical medication. Methods Real time fluorescent quantitative polymerase chain reaction(FQ-PCR)was used to detect Uroplasma urealytica(Uu),as well as the cultivation and drug sensitivity kit was used to cultivate and test the susceptibility of Uroplasma urealytica and Mycoplasma homminis(Mh). Results In 1,298 specimens Uu was detected in 670 cases by FQ-PCR and 487 cases by culture,Mh was detected in 13 cases,Mh and Uu mixed infection was detected in 42 cases by culture.The coincidence rate of both the two methods was 78.9%.The results of drug susceptibility showed the sensitive rates to deoxycycline and minocyclin were higher than other antibiotics in the single Uu infection,which were 81.3% and 80.6% respectively.The sensitive rates of deoxycycline and clarithromycin were high in single infection of Mh.However,the mixed infection of Uu and Mh showed multiple antibiotic resistance. Conclusion The prevalence of genitourinary tract mycoplasma infection is high in Shaoyang district.The combination of FQ-PCR and cultivation showed practical value in diagnosis and treatment of mycoplasma infection.Deoxycycline can be used as the first choice to anti-mycoplasma agent in our area,while combined usage of antibiotic should be considered in mixed infection.更多还原