【摘要】 目的:观察转化生长因子β1(TGF-β1)在脂多糖(LPS)致大鼠急性肺损伤(ALI)早期模型肺组织的表达情况,探讨其在ALI发病机制中的作用。方法:将27只SD大鼠随机分成3组,其中NS对照组为气管内滴注生理盐水12 h后取材,LPS1组、LPS2组为气管内滴注LPS 1.0 ml/kg(浓度为100μg/ml)后分别于12 h,24 h取材。用免疫组织化学方法测定肺组织TGF-β1的表达。结果:LPS2组大鼠肺组织TGF-β1的表达(140.081±21.854)较LPS1组(45.157±29.443)明显升高(P<0.05),并且两个损伤组(LPS1组、LPS2组)TGF-β1的表达均显著高于NS对照组(7.175±3.686)(P<0.05)。肺组织HE染色显示肺损伤24 h与12 h相比肺泡间隔增厚,间质增生,淋巴细胞、巨噬细胞及成纤维细胞浸润等表现明显增强。结论:ALI早期,TGF-β1诱导胶原合成可能在ALI发病机制中扮演重要角色。更多还原

【Abstract】 Objective:To observe transforming growth factor-β1（TGF-β1）expression on lung tissue in early fibroproliferation of acute lung injury（ALI） caused by Lipopolysacharide（LPS）,to explore the function of TGF-β1 in ALI.Methods:Twenty-seven SD rats were divided into 3 groups randomly,the NS group was instilled saline into trachea for 12 h;and the lung sample were made.The LPS1group and LPS2 group were instilled LPS 1.0 ml/kg（100 μg/ ml）for 12 h and 24 h respectively,and the lung sample were made.TGF-β₁ expression in lung was detected by immuohistochemistry.Results:Lung tissue TGF-β₁expression in LPS2 group（140.081±21.854）is significantly higher than that in LPS1（45.157±29.443）（P<0.05）.The TGF-β1 expression in the two injured groups（LPS1,LPS2）are significantly higher than that in NS group（7.175±3.686）（P<0.05）.The pulmonary section stained with HE revealed the interalveolar markedly thicken,interstitial proliferation,and immersed by lymphocytes,macrophage,fibroblast.And such revealment are more significant after 24 h than after 12 h.Conclusions:The TGF-β₁ perheps has important function in inducing the collagen synthesis in early ALI.The TGFβ₁ play an important role in the occurrence of ALI.更多还原