【摘要】 目的研究原花青素对佐剂性关节炎大鼠体内PGE2合成的抑制机理。方法建立大鼠佐剂性关节炎模型,EIA法测大鼠足爪炎症组织中PGE2的含量,RT PCR和Western blot分别检测大鼠腹腔巨噬细胞内COX 2 mRNA和蛋白的表达。结果原花青素显著降低佐剂性关节炎大鼠足爪炎症组织中的PGE2含量,下调大鼠腹腔巨噬细胞COX 2 mRNA和蛋白的表达。结论原花青素对佐剂性关节炎大鼠体内PGE2合成的抑制作用可能与其抑制COX 2 mRNA和蛋白表达有关。更多还原

【Abstract】 Objective To study the inhibition mechanism of proanthocyanidins on the PGE₂ product of rats with adjuvant arthritis（AA）.Methods The adjuvant arthritis model of rats was induced by complete freunds adjuvant.The contents of PGE₂ in paws of rats were measured by EIA.The expression of COX-2 mRNA and protein in rat′s peritoneal macrophages was analysed by RT-PCR and Western blot.Results Proanthocyanidins could significantly suppress the contents of PGE₂ in edema paws and inhibit the expression of COX-2 mRNA and protein in peritoneal macrophages of rats suffered with adjuvant arthritis.Conclusions Inhibition mechanism of proanthocyanidins on the PGE₂ product of rats with adjuvant arthritis may be related to inhibiting the expression of COX-2 mRNA and protein.更多还原