【摘要】 目的测定双硫仑羟丙基β环糊精(DSFβCDHP)的包合常数;研究包合物形成的热力学性质。方法采用RP HPLC法。测定溶液中双硫仑含量,由测定结果计算各浓度的βCDHP溶液中双硫仑的溶解度,以双硫仑溶解度对βCDHP浓度作图,得到相溶解度图;测定不同色谱条件下双硫仑羟丙基β环糊精的保留时间,根据保留时间计算稳定常数及不同温度下的包合常数K。结果当羟丙基β环糊精浓度较低时,可形成1∶1包合物;30℃时DSFβCDHP的包合常数为1 624.41 L.mol-1;其包合反应的焓变(ΔH)为-25.25 kJ.mol-1,吉布斯自由能(ΔG)和熵变(ΔS)分别为-12.60 kJ.mol-1与-41.74 J.mol-1K-1。结论双硫仑与羟丙基β环糊精的包合作用为一放热过程,是分子间氢键和范德华力作用的结果;采用较低的温度有利于双硫仑完全形成包合物。更多还原

【Abstract】 Objective To study the thermodynamic properties of disulfiram-hydroxypropyl-β-cyclodextrin(DSF-β-CD_HP) inclusion complexes and determine the equilibrium constants of the inclusion complex.Methods The reverse-phase high performance liquid chromatogram was employed to determine the retention times of DSF-β-CD_HP under different conditions,then the equilibrium constants and thermodynamic parameters were calculated.Results The inclusion complexes containing equimolar disulfiram（DSF） and hydroxypropyl-β-cyclodextrin(β-CD_HP) were formed when the concentration of β-CD_HP was low.The equilibrium constant of inclusion complexes was confirmed to be 1 624.41 L·mol^-1 at 30 ℃ through the way of RP-HPLC.The changes of enthalpy（ΔH⁰）,Gibbs’ free energy（ΔG⁰） and entropy（ΔS⁰） of the system during the formation of DSFβ-CD_HP were-25.25 kJ·mol^-1,(-12.60 kJ·mol^-1) and41.74 J·mol^-1K^-1 respectively.Conclusions The negative free energy change of this reaction demonstrate the spontaneous formation of DSF-β-CD_HP,which caused by the intermolecular hydrogen bond and Van der Waals forces.The low temperature is useful for the formation of inclusion complexes.更多还原