【摘要】 目的建立HPLC法测定辽东木根皮中木皂苷C含量的方法。方法固定相为DiamonsinC18柱(250 mm×4.6 mm,5μm),流动相为甲醇水磷酸(体积比为67.0∶33.0∶0.2),流速为0.8 mL.min-1,检测波长为206 nm,柱温为30℃。结果木皂苷C在20~40μg内呈良好的线性关系(r=0.999 9),平均回收率为99.7%,RSD为3.2%(n=6)。结论可用于木皂苷C的定量分析。更多还原

【Abstract】 Objective To develop the analysis method for the determination of the content of araloside C in the root bark of Aralia elata（Miq.）Seem.by HPLC.Methods The chromatographic condition was Diamonsin C₁₈ column（250 mm×4.6 mm,5 μm）.A mixture of methanol-water-phosphoric acid（V∶V∶V=（63.0∶）37.0∶0.2）was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was set at 206 nm at 30 ℃.Results The araloside C curve showed good linear correlation within the concentration range of（20～）40 μg（r=0.999 9）.The average recovery and RSD was 99.7% and 3.2%（（n=6）） respectively.Conclusions The method is convenient,reliable and with good reappearance.更多还原